Giuseppe Tempos

Giuseppe Cannone

CO-FOUNDER & CRYO-EM SPECIALIST

Meet Giuseppe, a seasoned electron microscopy expert with a decade of experience. He earned his PhD at Edinburgh University, focusing on DNA replicative machines and CRISPR in Archaea. Currently, Giuseppe serves as a Research Support Officer at MRC-Laboratory of Molecular Biology in Cambridge, UK, where he contributes to cutting-edge scientific research through user training, data collection advice, and microscope maintenance.

McMullan G, Naydenova K, Mihaylov D, Yamashita K, Peet MJ, Wilson H, Dickerson JL, Chen S, Cannone G, Lee Y, Hutchings KA, Gittins O, Sobhy MA, Wells T, El-Gomati MM, Dalby J, Meffert M, Schulze-Briese C, Henderson R, Russo CJ. 

Structure determination by cryoEM at 100 keV.

Proc Natl Acad Sci U S A. 2023 Dec 5;120(49):e2312905120

2021

Jenkyn-Bedford M, Jones ML, Baris Y, Labib KPM, Cannone G, Yeeles JTP, Deegan TD.

A conserved mechanism for regulating replisome disassembly in eukaryotes.

Cell Rep. 36(9):109648

Nasrallah C, Cannone G, Briot J, Rottier K, Berizzi AE, Huang CY, Quast RB, Hoh F, Banères JL, Malhaire F, Berto L, Dumazer A, Font-Ingles J, Gómez-Santacana X, Catena J, Kniazeff J, Goudet C, Llebaria A, Pin JP, Vinothkumar KR, Lebon G.

Agonists and allosteric modulators promote signaling from different metabotropic glutamate receptor 5 conformations.

Nature. 600(7890):743-747

2020

Baretić D, Jenkyn-Bedford M, Aria V, Cannone G, Skehel M, Yeeles JTP.

Cryo-EM structure of the fork protection complex bound to CMG at a replication fork.

Mol Cell. 78(5):926-940

2019

Sathyanarayanan N, Cannone G, Gakhar L, Katagihallimath N, Sowdhamini R, Ramaswamy S, Vinothkumar KR.

Molecular basis for metabolite channeling in a ring opening enzyme of the phenylacetate degradation pathway.

Nat Commun. 10(1):4127

Deng X, Gonzalez Llamazares A, Wagstaff JM, Hale VL, Cannone G, McLaughlin SH, Kureisaite-Ciziene D, Löwe J.

The structure of bactofilin filaments reveals their mode of membrane binding and lack of polarity.

Nat Microbiol. 4(12):2357-2368

2016

D'Este F, Benincasa M, Cannone G, Furlan M, Scarsini M, Volpatti D, Gennaro R, Tossi A, Skerlavaj B, Scocchi M.

Antimicrobial and host cell-directed activities of Gly/Ser-rich peptides from salmonid cathelicidins.

Fish Shellfish Immunol. 59:456-468

Rouillon C, Zhou M, Zhang J, Politis A, Beilsten-Edmands V, Cannone G, Graham S, Robinson CV, Spagnolo L, White MF.

Structure of the CRISPR interference complex CSM reveals key similarities with cascade.

Mol Cell. 52(1):124-134

Froese DS, Forouhar F, Tran TH, Vollmar M, Kim YS, Lew S, Neely H, Seetharaman J, Shen Y, Xiao R, Acton TB, Everett JK, Cannone G, Puranik S, Savitsky P, Krojer T, Pilka ES, Kiyani W, Lee WH, Marsden BD, von Delft F, Allerston CK, Spagnolo L, Gileadi O, Montelione GT, Oppermann U, Yue WW, Tong L.

Crystal structures of malonyl-coenzyme A decarboxylase provide insights into its catalytic mechanism and disease-causing mutations.

Structure. 21(7):1182-1192

2012

Zhang J, Rouillon C, Kerou M, Reeks J, Brugger K, Graham S, Reimann J, Cannone G, Liu H, Albers SV, Naismith JH, Spagnolo L, White MF.

Structure and mechanism of the CMR complex for CRISPR-mediated antiviral immunity.

Mol Cell. 45(3):303-313